
Perturbation effect is not an on-off switch
In this blog, we examine how the “perturbation effect” can vary depending on the metrics used to define it, and why these differences matter. While these metrics may appear interchangeable, they often capture fundamentally different aspects of the underlying biology. As Perturb-seq datasets continue to grow exponentially, understanding how perturbation effects are measured becomes critical for reliable downstream analysis. When suppression is not an on-off switch In 2025, Nadig and colleagues















